An altercation between two males occurred sometime Friday evening at an address on Anne Street.
A 45-year-old Barrie male succumbed to his injuries shortly after 8 p.m. on Saturday.
Investigators are looking to speak to 37-year-old Bryan Smith of Barrie. Police believe he is currently in the Midland-Penetang area and are urging him to contact investigators immediately.
The victim and suspect were known to each other and police do not believe there is a concern for public safety at this time.
If you have any information please contact Barrie Police Homicide Services at 705-725-7025 ext. 2160